Antibody and Dye Based Assays
Cell Signaling Technology MitoTracker Green FM, Live Cell Imaging, 500 μg
MitoTracker™ Green FM is a cell-permeable probe designed for labeling mitochondria in live cells. It contains a mildly thiol-reactive chloromethyl moiety that allows the dye to accumulate in active mitochondria by diffusing across the plasma membrane. It is specifically optimized for live-cell imaging; subsequent fixation with aldehydes or alcohols will inhibit staining.
- Excitation and emission wavelengths of 490 nm and 516 nm, respectively.
- Supplied as 50 μg lyophilized solid per vial.
- Reconstitute with 74.4 μl high-quality DMSO to produce a 1 mM stock solution.
- Recommended staining concentration is 100-400 nM with incubation for 15-30 min at 37°C.
- Compatible with phenol red-free media to minimize background fluorescence.
- Suitable for immunofluorescence and flow cytometry applications.
Cell Signaling Technology MitoTracker Red CMXRos, Cellular Dye, 500 μg
MitoTracker™ Red CMXRos is a cell-permeable probe containing a mildly thiol-reactive chloromethyl moiety designed for mitochondrial labeling. It is well-retained after fixation, allowing for subsequent sample processing and immunostaining. The dye passively diffuses across the plasma membrane and accumulates in active mitochondria.
- Excitation: 579 nm; Emission: 599 nm
- Molecular weight: 531.52
- Validated applications include immunofluorescence (immunocytochemistry) and flow cytometry
- Retained after fixation with ice-cold methanol
- Species reactivity: all species expected
- Store lyophilized solid at -20°C, desiccated and protected from light
- Reconstitute in DMSO to create a 1 mM stock solution
- Typical staining concentration: 50-200 nM
- Optimal incubation: 15-45 min at 37°C
ILEX LIFE SCIENCES LLC QuickZyme Hydroxyproline Assay Kit, 96 Assays, 96-well plate format, colorimetric assay for collagen analysis (conditioned media cells, cell extract, tissue, tissue extract samples)
The QuickZyme Hydroxyproline Assay Kit enables quantitative collagen analysis in all types of tissues, in a fast and simple 96-well plate format. This colorimetric assay does not need the drying step following acid hydrolysis of collagen for which often special equipment is needed. This assay is the only hydroxyproline-based assay that does not need this laborious step, resulting in a fast, sensitive, and easy assay. NOTE: This kit does not contain tubes or a protocol for collagen hydrolysis. If you need these items please order the QuickZyme Total Collagen Assay. Application: conditioned media cells, cell extracts, tissues, tissue extracts. Sensitivity: 2.4 µM (0.3 µg/ml). Assay-time: < 2 hours. Collagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Thus measurement of collagen production is important in many disease related studies.
Biotium Calnexin (Endoplasmic Reticulum Marker) (CANX/1541), CF594 conjugate, 0.1mg/mL
Calnexin is a type-I integral membrane protein of the endoplasmic reticulum (ER). It is a calcium-binding chaperone protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium Ep-CAM / CD326 (Rat)(GZ-20), CF488A conjugate, 0.1mg/mL
Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on basolateral cell surface in most simple epithelia and a vast majority of carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium CD81 / TAPA-1 (C81/2885R), CF488A conjugate, 0.1mg/mL
This antibody recognizes a protein of 26 kDa, identified as CD81 (Workshop VI; Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspanin transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness an
Chondrex Inc MOUSE ANTI-LPS IGG ASSAY KT
Assay kit to determine mouse anti-lipopolysaccharide (LPS) IgG antibodies. Kit comes with 96-well ELISA plate pre-coated with LPS (from E. coli O111:B4) and all reagents necessary to complete the assay. Standard Range: 100 ng/mL to 1.6 ng/mL. Working Time: 4.5 Hours.
Biotium Calnexin (Endoplasmic Reticulum Marker) (CANX/1543), Biotin conjugate, 0.1mg/mL
Calnexin is a type-I integral membrane protein of the endoplasmic reticulum (ER). It is a calcium-binding chaperone protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Medchemexpress LLC (E)-9-amino-10-methyl-2-(naphthalen-1-ylmethylene)-1-oxo-1,2,3,4-tetrahydroacridin-10-ium iodide | 2252162-81-3 | 98.1% | 492.35 g/mol | C25H21IN2O | 5 MG
Aβ/tau aggregation-IN-1 is a small-molecule research compound that inhibits aggregation of amyloid-β (Aβ1-42) and tau proteins. It reduces β-sheet formation through noncovalent interactions, has reported brain permeability, and is supplied with documented solubility and storage recommendations for both in vitro and in vivo use.
- Inhibits Aβ1-42 and tau aggregation in biochemical assays.
- Reduces β-sheet formation via noncovalent interactions.
- Reported to be brain permeable for central nervous system studies.
- Suitable for in vitro and in vivo research applications.
- High purity compound (reported >98%).
- Soluble in DMSO; documented in vivo formulation options yield ≥1 mg/mL.
- Store powder at 4°C; store solutions at -80°C (up to 6 months) or -20°C (up to 1 month).
Infab Corporation Infab Lightning Front Protection Radiation Protection Apron
LIGHTNING FRONT PROTECTION APRON, SIZE L, KIARMOR LEAD FREE, .50MM, COLOR BLACK.
Infab Corporation Infab Lightning Front Protection Radiation Protection Apron
LIGHTNING FRONT PROTECTION APRON, SIZE M, KIARMOR LEAD FREE, .50MM, COLOR BLACK.
Medchemexpress LLC Aβ/tau aggregation-IN-1 | 2252162-81-3 | 98.1% | C25H21IN2O | 100 MG
Aβ/tau aggregation-IN-1 is a potent inhibitor of Aβ1-42 β-sheets formation and tau aggregation. It has KD values of 160 μM with Aβ1-42 and 337 μM with tau. This compound can permeate the blood-brain barrier. It significantly declines tau aggregation foci, effectively inhibiting Aβ1-42 aggregation by 84.7%-99.5% and tau aggregation by 71.2%-101.8%.
- Inhibits Aβ1-42 β-sheets formation
- Inhibits tau aggregation
- Permeates the blood-brain barrier
- Declines tau aggregation foci
- Inhibits self-induced Aβ1-42 aggregation
- Shows binding abilities with Aβ1-42 and tau
Biotium CD48(156-4H9), CF488A conjugate, 0.1mg/mL
Reacts with human CD48, a 45 kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.